HSP60 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31750
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31750-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HSP60 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of HSP60 antibody and Lane 1: rat kidney; 2: mouse kidney; 3: (m) HEPA; 4: (r) NRK; 5: (r) PC12; 6: human SW620; 7: (h) A549; 8: (h) A431; 9: (h) HeLa lysate. Predicted/observed size ~60KD
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IHC-P: HSP60 antibody testing of human intestine cancer tissue. HIER: steamed with pH6 citrate buffer.
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ICC testing of HSP60 antibody and SMMC-7721 cells.
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IHC-P testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P testing of rat intestine tissue. HIER: steamed with pH6 citrate buffer.
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Immunofluorescent staining of FFPE human U-2 OS cells with HSP60 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human A431 cells with HSP60 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSP60 antibody.
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Western blot testing of HSP60 antibody and Lane 1: rat kidney; 2: mouse kidney; 3: (m) HEPA; 4: (r) NRK; 5: (r) PC12; 6: human SW620; 7: (h) A549; 8: (h) A431; 9: (h) HeLa lysate. Predicted/observed size ~60KD
IHC-P: HSP60 antibody testing of human intestine cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of HSP60 antibody and SMMC-7721 cells.
IHC-P testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
IHC-P testing of rat intestine tissue. HIER: steamed with pH6 citrate buffer.
Immunofluorescent staining of FFPE human U-2 OS cells with HSP60 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A431 cells with HSP60 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HSP60 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (Paraffin): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 2-3ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the HSP60 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2signaling.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
3329
Immunogen:
Human partial recombinant protein (AA 260-496) was used as the immunogen for this HSP60 antibody.
Limitation:
This HSP60 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat