HSP70 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30043
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30043-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: BRM-22, aka SJ-70
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
  • Rabbit
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HSP70 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human breast cancer tissue with HSP70 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.~
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Western blot testing of 1) human MCF7, 2) human 293T, 3) human A549, 4) human U-2 OS, 5) rat
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IHC staining of FFPE human breast cancer tissue with HSP70 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.~
Western blot testing of 1) human MCF7, 2) human 293T, 3) human A549, 4) human U-2 OS, 5) rat

Documents

Further Information

Application Details :
Western blot: 0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml, formalin or acetone fixed tissues
Application Note:
The stated application concentrations are suggested starting points. Titration of the HSP70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
3303
Immunogen:
Heat shock protein 70 isolated from bovine brain was used as the immunogen for this HSP70 antibody.
Limitation:
This HSP70 antibody is available for research use only.
Purity:
Ascites
Species Reactivity :
Human, rat, rabbit, bovine