HSP90 Antibody / HSP90AA1

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NSJ Bioreagents
Product Code: NSJ-R31515
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31515-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HSP90 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Hsp90 antibody and Lane 1: rat liver; 2: HeLa; 3: MCF-7; 4: Raji lysate. Predicted molecular weight: ~83kDa, routinely observed at 83-90kDa.
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IHC-P: HSP90 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
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ICC testing of HSP90 antibody and MCF-7 cells
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ICC testing of HSP90 antibody and A549 cells
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IHC-P: HSP90 antibody testing of mouse brain tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P: HSP90 antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
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Western blot testing of Hsp90 antibody and Lane 1: rat liver; 2: HeLa; 3: MCF-7; 4: Raji lysate. Predicted molecular weight: ~83kDa, routinely observed at 83-90kDa.
IHC-P: HSP90 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of HSP90 antibody and MCF-7 cells
ICC testing of HSP90 antibody and A549 cells
IHC-P: HSP90 antibody testing of mouse brain tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: HSP90 antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the HSP90 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Heat shock protein 90 is a chaperone protein that assists other proteins to fold properly, stabilizes proteins against heat stress, and aids in protein degradation. Inhibitors of it are investigated as anti-cancer drugs. Heat shock proteins, as a class, are among the most highly expressed cellular proteins across all species. HSP90 acts as a capacitor for morphologic evolution through epigenetic and genetic mechanisms. It is a molecular chaperone that plays a key role in the conformational maturation of oncogenic signaling proteins, including HER2/ERBB2, AKT, RAF1, BCR-ABL, and mutated p53. Although it is highly expressed in most cells, HSP90 inhibitors selectively kill cancer cells compared to normal cells, and the the inhibitor 17-allylaminogeldanamycin (17-AAG) exhibited antitumor activity in preclinical models.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
3320
Immunogen:
Human partial recombinant protein (AA 2-365) was used as the immunogen for this HSP90 antibody.
Limitation:
This HSP90 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat