HSPB1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30899
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30899-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the HSPB1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human HeLa, 2) monkey COS-7, 3) human Caco-2 and 4) human Jurkat cell lysate with HSPB1 antibody. Expected molecular weight: 23~27 kDa.
2 / 4
ICC staining of FFPE human A549 cells with HSPB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 4
IHC staining of FFPE human lung cancer with HSPB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
4 / 4
IHC staining of FFPE rat heart with HSPB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Western blot testing of 1) human HeLa, 2) monkey COS-7, 3) human Caco-2 and 4) human Jurkat cell lysate with HSPB1 antibody. Expected molecular weight: 23~27 kDa.
ICC staining of FFPE human A549 cells with HSPB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human lung cancer with HSPB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE rat heart with HSPB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the HSPB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HSPB1 (Heat shock 27kDa protein 1) is a protein that in humans is encoded by the HSPB1 gene. Stock et al.(2003) used FISH to map the HSP27 gene to 7q11.23. The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human HSP27 (KHEERQDEHGYISRCFTR) was used as the immunogen for this HSPB1 antibody (100% homologous in human, mouse and rat).
Limitation:
This HSPB1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P04792