HSPB2 Antibody

Western blot: 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the HSPB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al.(1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They called it Heat shock 27 protein 2 or Heat shock protein beta 2 and termed the gene HSPB2. Iwaki et al.(1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2 is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYABgene is highly expressed in all three tissues. The two related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the middle region of human HSPB2 (RHPQRLDRHGFVSREFC) was used as the immunogen for this HSPB2 antibody (100% homologous in human, mouse and rat).

This HSPB2 antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

Q16082