Human IL3 Antibody

Western blot (recombinant protein): 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml

The stated application concentrations are suggested starting amounts. Titration of the human IL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 The mouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Human partial recombinant protein (AA 20-152) was used as the immunogen for this human IL3 antibody.

This human IL3 antibody is available for research use only.

Antigen affinity

Human

P08700