ICAM1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4001
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4001-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the ICAM1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of human 1) Raji, 2) K562 and 3) HepG2 cell lysate with ICAM1 antibody at 0.5ug/ml. Predicted molecular weight: ~58 kDa (unmodified), 75-115 kDa (glycosylated).
2 / 6
Western blot testing of rat 1) spleen, 2) thymus and 3) RH35 lysate and mouse 4) spleen, 5) thymus, 6) HEPA1-6 and 7) heart lysate with ICAM1 antibody at 0.5ug/ml. Predicted molecular weight: ~58 kDa (unmodified), 75-115 kDa (glycosylated).
3 / 6
Immunofluorescent staining of FFPE human A431 cells with ICAM1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 6
Flow cytometry testing of human PC-3 cells with ICAM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ICAM1 antibody.
5 / 6
IHC staining of FFPE human lung cancer with ICAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 6
IHC staining of FFPE human brest cancer with ICAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of human 1) Raji, 2) K562 and 3) HepG2 cell lysate with ICAM1 antibody at 0.5ug/ml. Predicted molecular weight: ~58 kDa (unmodified), 75-115 kDa (glycosylated).
Western blot testing of rat 1) spleen, 2) thymus and 3) RH35 lysate and mouse 4) spleen, 5) thymus, 6) HEPA1-6 and 7) heart lysate with ICAM1 antibody at 0.5ug/ml. Predicted molecular weight: ~58 kDa (unmodified), 75-115 kDa (glycosylated).
Immunofluorescent staining of FFPE human A431 cells with ICAM1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human PC-3 cells with ICAM1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ICAM1 antibody.
IHC staining of FFPE human lung cancer with ICAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brest cancer with ICAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ICAM1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CD54, also known as Intercellular adhesion molecule-1 (ICAM1) is a ligand for lymphocyte function-associated (LFA) antigens. ICAM-1 is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for LFA-1, a beta 2 leukocyte integrin. This protein is the major human rhinovirus receptor. The ICAM1 gene is mapped to human chromosome 19. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer CD11a/CD18 (Leu-CAMa, LFA-1) and its ligand CD54 (ICAM-1).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids Q28-R268 was used as the immunogen for the ICAM1 antibody.
Limitation:
This ICAM1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P05362