ICAM-1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31450
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31450-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ICAM-1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of ICAM-1 antibody and Lane 1: HEPG2; 2: K562; 3: A549; 4: HT1080 lysate. The glycoprotein is routinely visualized at 75~115KD.
2 / 5
IHC-P: ICAM-1 antibody testing of human intestinal cancer tissue
3 / 5
Immunofluorescent staining of FFPE human tonsil tissue with ICAM-1 antibody (green) at 1ug/ml and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 5
Immunofluorescent staining of FFPE human lung cancer with ICAM-1 antibody (green) at 1ug/ml and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 5
Flow cytometry testing of human A431 cells with ICAM-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ICAM-1 antibody.
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Western blot testing of ICAM-1 antibody and Lane 1: HEPG2; 2: K562; 3: A549; 4: HT1080 lysate. The glycoprotein is routinely visualized at 75~115KD.
IHC-P: ICAM-1 antibody testing of human intestinal cancer tissue
Immunofluorescent staining of FFPE human tonsil tissue with ICAM-1 antibody (green) at 1ug/ml and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human lung cancer with ICAM-1 antibody (green) at 1ug/ml and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with ICAM-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ICAM-1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the ICAM-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Intercellular adhesion molecule-1 is a ligand for LFA-1, a beta 2 leukocyte integrin. In humans, lymphocyte adhesion to cells is mediated by the protein heterodimer Leu-CAMa/LFA-1 and its ligand ICAM-1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
3383
Immunogen:
Human partial recombinant protein (AA 214-532) was used as the immunogen for this ICAM-1 antibody.
Limitation:
This ICAM-1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human