IDE Antibody / Insulin degrading enzyme

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4291
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4291-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the IDE antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of human 1) HeLa, 2) placenta, 3) COLO-320, 4) HepG2, 5) SGC-7901, 6) Jurkat, 7) rat stomach, 8) mouse skeletal muscle, 9) mouse stomach and 10) mouse brain lysate with IDE antibody at 0.5ug/ml. Predicted molecular weight ~118 kDa.
2 / 5
IHC testing of FFPE human breast cancer tissue with IDE antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
IHC testing of FFPE rat pancreas tissue with IDE antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
Flow cytometry testing of human HeLa cells with IDE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=IDE antibody.
5 / 5
Flow cytometry testing of human Caco-2 cells with IDE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=IDE antibody.
❮❯

Western blot testing of human 1) HeLa, 2) placenta, 3) COLO-320, 4) HepG2, 5) SGC-7901, 6) Jurkat, 7) rat stomach, 8) mouse skeletal muscle, 9) mouse stomach and 10) mouse brain lysate with IDE antibody at 0.5ug/ml. Predicted molecular weight ~118 kDa.
IHC testing of FFPE human breast cancer tissue with IDE antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat pancreas tissue with IDE antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HeLa cells with IDE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=IDE antibody.
Flow cytometry testing of human Caco-2 cells with IDE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=IDE antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml, IHC (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the IDE antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Insulin-degrading enzyme, also known as IDE, is an enzyme. This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids F485-K756 was used as the immunogen for the IDE antibody.
Limitation:
This IDE antibody is available for research use only.
Localization:
Cytoplasm, cell membrane, secreted
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14735