IGFBP1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32872
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32872-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the IGFBP1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE mouse liver tissue with IGFBP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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IHC testing of FFPE mouse pancreas tissue with IGFBP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 6
IHC testing of FFPE rat liver tissue with IGFBP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 6
IHC testing of FFPE rat pancreas tissue with IGFBP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 6
Western blot testing of recombinant mouse protein with IGFBP1 antibody at 0.5ug/ml. Expected molecular weight: 28-35 kDa.
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Western blot testing of 1) rat liver and 2) mouse liver lysate with IGFBP1 antibody. Expected molecular weight: 28-35 kDa.
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IHC testing of FFPE mouse liver tissue with IGFBP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse pancreas tissue with IGFBP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat liver tissue with IGFBP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat pancreas tissue with IGFBP1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of recombinant mouse protein with IGFBP1 antibody at 0.5ug/ml. Expected molecular weight: 28-35 kDa.
Western blot testing of 1) rat liver and 2) mouse liver lysate with IGFBP1 antibody. Expected molecular weight: 28-35 kDa.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,ELISA (Capture; recombinant mouse protein): 0.1-0.5ug/ml (BSA-free format available)
Application Note:
Optimal dilution of the IGFBP1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
IGFBP1, Insulin-like growth factor-binding protein 1, also known as placental protein 12 (PP12), is a protein that in humans is encoded by the IGFBP1 gene. The gene has 4 exons and spans 5.9 kb. And the IGFBP1 gene is localized to 7p13-p12 by in situ hybridization. This gene is a member of the Insulin-like growth factor-binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a type-I thyroglobulin domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse protein corresponding to amino acids A26-N272 was used as the immunogen for the IGFBP1 antibody.
Limitation:
This IGFBP1 antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
P47876