IKK alpha Antibody

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NSJ Bioreagents
Product Code: NSJ-R31535
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31535-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the IKK alpha antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human lung cancer tissue with IKK alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.~
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Western blot testing of human 1) placenta, 2) K562, 3) HepG2 and 4) Caco-2 lysate with IKK
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IHC staining of FFPE human lung cancer tissue with IKK alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.~
Western blot testing of human 1) placenta, 2) K562, 3) HepG2 and 4) Caco-2 lysate with IKK

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the IKK alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Inhibitor of nuclear factor kappa-B kinase subunit alpha, also known as conserved helix-loop-helix ubiquitous kinase (CHUK) or IKBKA, is a protein kinase that in humans is encoded by the CHUK gene. It is mapped to 10q24.31. This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. IKK alpha is part of the IkB kinase complex that plays an important role in regulating the NF-kB transcription factor. IKK alpha also has many additional cellular targets, and is thought to function independently of the NF-kB pathway to regulate epidermal differentiation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
1147
Immunogen:
Human partial recombinant protein (AA 411-745) was used as the immunogen for this IKK alpha antibody.
Limitation:
This IKK alpha antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human