IKK gamma Antibody / NEMO

Contact us
NSJ Bioreagents
Product Code: NSJ-R32596
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32596-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the IKKG antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with IKKG antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Flow cytometry testing of human U-87 MG cells with IKKG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IKKG antibody.
3 / 4
Flow cytometry testing of human PC-3 cells with IKKG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IKKG antibody.
4 / 4
Western blot testing of 1) rat heart and 2) human HepG2 lysate with IKKG antibody at 0.5ug/ml. Expected molecular weight: 37-60 kDa.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with IKKG antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-87 MG cells with IKKG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IKKG antibody.
Flow cytometry testing of human PC-3 cells with IKKG antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IKKG antibody.
Western blot testing of 1) rat heart and 2) human HepG2 lysate with IKKG antibody at 0.5ug/ml. Expected molecular weight: 37-60 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml
Application Note:
Optimal dilution of the IKKG antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
NF-kappa-B essential modulator (NEMO), also known as inhibitor of nuclear factor kappa-B kinase subunit gamma (IKKg), is a protein that in humans is encoded by the IKBKG gene. This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 207-246 (QSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIK-human) were used as the immunogen for the IKKG antibody.
Limitation:
This antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9Y6K9