IKKb Antibody

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NSJ Bioreagents
Product Code: NSJ-R31634
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31634-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the IKKb antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with IKKb antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
Western blot testing of IKKb antibody and recombinant human protein (0.5ng)
3 / 5
IHC-P: IKKb antibody testing of human lung cancer tissue
4 / 5
Flow cytometry testing of human 293T cells with IKKb antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IKKb antibody.
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Western blot testing of IKKb antibody and human lysates- Lane 1: HEPG2; 2: COLO320; 3: M231; 4: HT1080 lysate. Expected molecular weight: ~87 kDa.
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Immunofluorescent staining of FFPE human A431 cells with IKKb antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of IKKb antibody and recombinant human protein (0.5ng)
IHC-P: IKKb antibody testing of human lung cancer tissue
Flow cytometry testing of human 293T cells with IKKb antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IKKb antibody.
Western blot testing of IKKb antibody and human lysates- Lane 1: HEPG2; 2: COLO320; 3: M231; 4: HT1080 lysate. Expected molecular weight: ~87 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the IKKb antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
I kappa B kinase beta, also known as IKKb or NFKBIKB, is a protein that in humans is encoded by the IKBKB gene.IkBabinds and inhibits NF-kB in the cytoplasm. Activated IKKb phosphorylates IkBa, which freesNFkBto translocate to the nucleus. It is found that mutations in IKKb had a more pronounced effect upon NFKB activation than did comparable mutations inIKKa.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
3551
Immunogen:
Human partial recombinant protein (AA 398-756) was used as the immunogen for this IKKb antibody.
Limitation:
This IKKb antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human