IL-18 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31447
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31447-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the IL-18 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE rat spleen with IL-18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of mouse 1) spleen, 2) thymus, 3) lung and 4) ANA-1 cell lysate with IL-18 antibody. Predicted molecular weight: 17~24 kDa.
3 / 3
Flow cytometry testing of rat RH-35 cells with IL-18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL-18 antibody.
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IHC staining of FFPE rat spleen with IL-18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse 1) spleen, 2) thymus, 3) lung and 4) ANA-1 cell lysate with IL-18 antibody. Predicted molecular weight: 17~24 kDa.
Flow cytometry testing of rat RH-35 cells with IL-18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL-18 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the IL-18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Interleukin-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL-18 is a cytokine that modulates both T helper type 1 (Th1) and Th2 responses. It is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
29197
Immunogen:
Rat partial recombinant protein (AA 37-194) was used as the immunogen for this IL-18 antibody.
Limitation:
This IL-18 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Rat, Mouse