IL-27 Antibody (alpha subunit)

NSJ Bioreagents
Product Code: NSJ-RQ4175
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4175-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the IL-27 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human CCRF-CEM cell lysate with IL-27 antibody at 0.5ug/ml. Predicted molecular weight ~28 kDa, observed here at ~20 kDa.

Western blot testing of human CCRF-CEM cell lysate with IL-27 antibody at 0.5ug/ml. Predicted molecular weight ~28 kDa, observed here at ~20 kDa.

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,ELISA (Capture): 0.1-0.5ug/ml
Application Note:
Optimal dilution of the IL-27 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Interleukin 27 (IL-27) is a member of the IL-12 cytokine family. The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids F29-P243 was used as the immunogen for the IL-27 antibody.
Limitation:
This IL-27 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q8NEV9