Il-33 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32998
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32998-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Il33 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
IHC testing of FFPE mouse spleen tissue with Il33 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
2 / 4
IHC testing of FFPE rat spleen tissue with Il33 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 4
IHC testing of FFPE rat lymph tissue with Il33 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 4
Western blot testing of rat 1) spleen and 2) lung tissue lysate with Il33 antibody at 0.5ug/ml. Predicted molecular weight ~31 kDa.
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IHC testing of FFPE mouse spleen tissue with Il33 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat spleen tissue with Il33 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat lymph tissue with Il33 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of rat 1) spleen and 2) lung tissue lysate with Il33 antibody at 0.5ug/ml. Predicted molecular weight ~31 kDa.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,ELISA (Capture): 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Il33 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Interleukin 33 (IL-33) is a protein that in humans is encoded by the IL33 gene. The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant rat partial protein corresponding to amino acids A102-M264 was used as the immunogen for the Il33 antibody.
Limitation:
This Il33 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic, secreted
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q66H70