Isg15 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32374
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32374-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Isg15 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of mouse 1) intestine and 2) spleen lysate with Isg15 antibody. Expected molecular weight: 15-17 kDa.
2 / 3
IHC testing of FFPE mouse kidney with Isg15 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
3 / 3
Western blot testing of mouse 1) spleen, 2) heart, 3) lung, 4) liver and 5) kidney tissue lysate with Isg15 antibody. Expected molecular weight: 15-17 kDa.
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Western blot testing of mouse 1) intestine and 2) spleen lysate with Isg15 antibody. Expected molecular weight: 15-17 kDa.
IHC testing of FFPE mouse kidney with Isg15 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Western blot testing of mouse 1) spleen, 2) heart, 3) lung, 4) liver and 5) kidney tissue lysate with Isg15 antibody. Expected molecular weight: 15-17 kDa.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the Isg15 antibody should be determined by the researcher.
Description:
Interferon-stimulated gene 15 (ISG15) is a 17 kDA secreted protein that in humans is encoded by the ISG15 gene. The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids A2-G155 from the mouse protein were used as the immunogen for the Isg15 antibody.
Limitation:
This Isg15 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Mouse
Uniprot #:
Q64339