ISL1 Antibody (Islet 1)

NSJ Bioreagents
Product Code: NSJ-R32744
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32744-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ISL1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat PC12 and 2) human MCF7 lysate with ISL1 antibody at 0.5ug/ml. Predicted molecular weight ~39 kDa.~
2 / 2
IHC testing of FFPE human breast cancer tissue with ISL1 antibody at 1ug/ml. Required HIER: steam section in pH6 citr

Western blot testing of 1) rat PC12 and 2) human MCF7 lysate with ISL1 antibody at 0.5ug/ml. Predicted molecular weight ~39 kDa.~
IHC testing of FFPE human breast cancer tissue with ISL1 antibody at 1ug/ml. Required HIER: steam section in pH6 citr

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the ISL1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Insulin gene enhancer protein ISL-1 is a protein that in humans is encoded by the isl1 gene. This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 118-161 (DEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAE) from the human protein were used as the immunogen for the ISL1 antibody.
Limitation:
This ISL1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P61371