ITGB4 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31440
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31440-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ITGB4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC-P: ITGB4 antibody testing of human intestinal cancer tissue
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IHC-P: ITGB4 antibody testing of human placenta tissue
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Western blot testing of ITGB4 antibody and recombinant human protein (0.5ng)
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Western blot testing of human 1) A549, 2) PC-3, 3) T-47D, 4) HeLa and 5) Caco-2 cell lysate with ITGB4 antibody at 0.5ug/ml. Predicted molecular weight ~200 kDa.
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Immunofluorescent staining of FFPE human placenta with ITGB4 antibody (green) at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Flow cytometry testing of human A431 cells with ITGB4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ITGB4 antibody.
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IHC-P: ITGB4 antibody testing of human intestinal cancer tissue
IHC-P: ITGB4 antibody testing of human placenta tissue
Western blot testing of ITGB4 antibody and recombinant human protein (0.5ng)
Western blot testing of human 1) A549, 2) PC-3, 3) T-47D, 4) HeLa and 5) Caco-2 cell lysate with ITGB4 antibody at 0.5ug/ml. Predicted molecular weight ~200 kDa.
Immunofluorescent staining of FFPE human placenta with ITGB4 antibody (green) at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with ITGB4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ITGB4 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Titration of the ITGB4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ITGB4, also known as CD104, is a receptor for the laminin proteins. It tends to associate with alpha 6 subunits and is likely to play a pivotal role in the biology of invasive carcinoma. It was found that ITGB4 was upregulated 6-fold by ZKSCAN3 in transfected human colon cancer cells compared with parental cells and ZKSCAN3 has been confirmed as the promoter of ITGB4 in vitro and in vivo. ITGB4 knockdown by short hairpin RNA countered ZKSCAN3-augmented anchorage-independent colony formation in the colon cancer cell lines.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Human partial recombinant protein (AA 28-266) was used as the immunogen for this ITGB4 antibody.
Limitation:
This ITGB4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P16144