Ki67 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31458
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31458-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Ki67 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of Ki67 antibody and Lane 1: HeLa; 2: MCF-7; 3: COLO320; 4: HEPG2; 5: SKOV lysate. Predicted/observed size ~350KD
2 / 4
IHC-P: Ki67 antibody testing of human tonsil tissue
3 / 4
Immunofluorescent staining of FFPE human colon tissue with Ki67 antibody (green) at 5ug/ml and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 4
Immunocytochemical staining of FFPE human HeLa cells with Ki67 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of Ki67 antibody and Lane 1: HeLa; 2: MCF-7; 3: COLO320; 4: HEPG2; 5: SKOV lysate. Predicted/observed size ~350KD
IHC-P: Ki67 antibody testing of human tonsil tissue
Immunofluorescent staining of FFPE human colon tissue with Ki67 antibody (green) at 5ug/ml and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunocytochemical staining of FFPE human HeLa cells with Ki67 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Proliferation-related Ki-67 antigen, also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
4288
Immunogen:
Human partial recombinant protein (AA 2860-3256) was used as the immunogen for this Ki67 antibody.
Limitation:
This Ki67 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human