KLF5 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30989
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30989-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the KLF5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa and 2) A549 cell lysate with KLF5 antibody. Expected molecular weight: 41-51 kDa (two isoforms).
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Western blot testing of human 1) HeLa and 2) A549 cell lysate with KLF5 antibody. Expected molecular weight: 41-51 kDa (two isoforms).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the KLF5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Kruppel-like factor 5 is a protein that in humans is encoded by the KLF5 gene. It is also known as BTEB2. This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Suske et al.(2005) stated that the human gene maps to chromosome 13q21.33, and the mouse gene to chromosome 14E2.1. Sogawa et al.(1993) isolated a cDNA encoding KLF5. The predicted protein contains 3 consecutive zinc finger motifs near the C terminus. The zinc finger domains were 59% and 64% identical to those of Sp1 (189906) and BTEB1, respectively. Both KLF5 and Sp1 have a short basic region preceding the zinc finger motifs that may play an auxiliary role in DNA binding. The N-terminal region of KLF5 is proline rich. Recombinant KLF5 protein and Sp1 showed very similar DNA-binding specificity in vitro. When expressed in mammalian cells, KLF5 activated the transcription of a reporter gene fused to a GC box-containing promoter. Sogawa et al.(1993) also identified the rat homolog.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human Kruppel-like factor 5 (HKKYRRDSASVVDQFFTD) was used as the immunogen for this KLF5 antibody (100% homologous in human, mouse and rat).
Limitation:
This KLF5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13887