KLF8 Antibody

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the KLF8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Kruppel-like factor 8 is a protein that in humans is encoded by the KLF8 gene. It is also named as ZNF741, or BKLF3. Kruppel-like factor proteins all contain a characteristic C-terminal DNA-binding domain containing three Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. The International Radiation Hybrid Mapping Consortium mapped the gene to the X chromosome (WI-18614). Suske et al.(2005) stated that the human gene maps to chromosome Xp11.21, and the mouse gene to chromosome XF3. van Vliet et al.(2000) identified a cDNA encoding KLF8, also called ZNF741. Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box. Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser(PVDLS) motif. Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the N-terminus of human Kruppel-like factor 8 (MVDMDKLINNLEVQLNSE) was used as the immunogen for this KLF8 antibody.

This KLF8 antibody is available for research use only.

Antigen affinity

Human

O95600