Lamin A Antibody

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NSJ Bioreagents
Product Code: NSJ-R31693
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31693-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Lamin A antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with Lamin A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE mouse NIH-3T3 cells with Lamin A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC-P: Lamin A antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P testing of rat intestine tissue. HIER: steamed with pH6 citrate buffer.
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Flow cytometry testing of human ThP-1 cells with Lamin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Lamin A antibody.
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Western blot testing of Lamin A antibody and Lane 1: human placenta; 2: SKOV; 3: SW620; 4: COLO320; 5: HeLa; 6: 293T; 7: A549 lysate. Predicted molecular weight: ~74 kDa.
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Immunofluorescent staining of FFPE human A431 cells with Lamin A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE mouse NIH-3T3 cells with Lamin A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: Lamin A antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
IHC-P testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
IHC-P testing of rat intestine tissue. HIER: steamed with pH6 citrate buffer.
Flow cytometry testing of human ThP-1 cells with Lamin A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Lamin A antibody.
Western blot testing of Lamin A antibody and Lane 1: human placenta; 2: SKOV; 3: SW620; 4: COLO320; 5: HeLa; 6: 293T; 7: A549 lysate. Predicted molecular weight: ~74 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the Lamin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The Lamin A/C (LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-Lamin A and C. Lamin A/C is mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
4000
Immunogen:
Human partial recombinant protein (AA 481-646) was used as the immunogen for this Lamin A antibody.
Limitation:
This Lamin A antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat