Lamin B1 Antibody

Product Code: NSJ-R32004

Code	Size	Price

NSJ-R32004-100ug

100 ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Immunocytochemistry (ICC)
Immunohistochemistry- Frozen Section (IHC-F)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the LMNB1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with LMNB1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

2 / 13

IHC testing of FFPE human breast cancer tissue with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

3 / 13

IHC testing of FFPE mouse intestine with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

4 / 13

IHC testing of FFPE rat intestine with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

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IHC testing of frozen human placental tissue with LMNB1 antibody.

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IHC testing of frozen mouse small intestine tissue with LMNB1 antibody.

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IHC testing of frozen rat small intestine tissue with LMNB1 antibody.

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ICC testing of FFPE human SW480 cells with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

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ICC testing of FFPE human SMMC-7721 cells with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

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ICC testing of FFPE human A549 cells with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

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ICC testing of FFPE human HeLa cells with LMNB1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

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Western blot testing of 1) human U87, 2) rat PC-12 and 3) mouse NIH3T3 lysate with LMNB1 antibody. Predicted molecular weight ~66 kDa.

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Flow cytometry testing of human U937 cells with LMNB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LMNB1 antibody.

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Documents

Further Information

Application Details :

Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells

Application Note:

Optimal dilution of the LMNB1 antibody should be determined by the researcher.

Description:

Lamin B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Format :

Antigen affinity purified

Formulation :

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Immunogen:

Amino acids 266-583 of human Lamin B1 were used as the immunogen for the LMNB1 antibody.

Limitation:

This LMNB1 antibody is available for research use only.

Localization:

Nuclear membrane, nuclear

Purity:

Antigen affinity

Species Reactivity :

Human, Mouse, Rat

Uniprot #:

P20700