Laminin Antibody (Gamma 1-3)

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NSJ Bioreagents
Product Code: NSJ-R32946
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32946-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Laminin gamma antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of 1) mouse lung, 2) rat heart, 3) rat lung and 4) human HeLa lysate with Laminin gamma antibody at 0.5ug/ml. Expected molecular weight: 172-200 kDa (G1 & G3), 130-140 kDa (G2).
2 / 5
IHC testing of FFPE mouse heart tissue with Laminin gamma antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 5
IHC testing of FFPE mouse kidney tissue with Laminin gamma antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 5
IHC testing of FFPE rat heart tissue with Laminin gamma antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 5
IHC testing of FFPE rat kidney tissue with Laminin gamma antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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Western blot testing of 1) mouse lung, 2) rat heart, 3) rat lung and 4) human HeLa lysate with Laminin gamma antibody at 0.5ug/ml. Expected molecular weight: 172-200 kDa (G1 & G3), 130-140 kDa (G2).
IHC testing of FFPE mouse heart tissue with Laminin gamma antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse kidney tissue with Laminin gamma antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat heart tissue with Laminin gamma antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat kidney tissue with Laminin gamma antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Laminin gamma antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an ?-chain, a ?-chain, and a ?-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acid sequences from Laminin gamma 1-3 (NKLNEIEGSLNKAKDEMKAS + DLEERVRRQRNHLHLLETSI + LQLDSHGALHHKLRQLEEES) were used as the immunogen for the Laminin gamma antibody.
Limitation:
This Laminin gamma antibody is available for research use only.
Localization:
Membrane, secreted (cytoplasmic in some tissue)
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P02468, Q61092, Q9R0B6