Laminin Antibody (Gamma)

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NSJ Bioreagents
Product Code: NSJ-R31563
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31563-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Laminin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Laminin antibody and Lane 1: HeLa; 2: HEPG2 lysate
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IHC staining of FFPE human lung cancer with Laminin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC staining of frozen human placenta with Laminin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of Laminin antibody and Lane 1: HeLa; 2: HEPG2 lysate
IHC staining of FFPE human lung cancer with Laminin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of frozen human placenta with Laminin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunohistochemistry (Frozen): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Laminin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Laminins are major proteins in the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. Laminins form independent networks and are associated with type IV collagen networks via entactin, fibronectin, and perlecan. They are important and biologically active parts of the basal lamina, influencing cell differentiation, migration, and adhesion, as well as phenotype and survival. Laminins are trimeric proteins that contain an alpha-chain, a beta-chain, and a gamma-chain, found in five, four, and three genetic variants, respectively. Laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
3915, 3918, 10319
Immunogen:
A peptide mixture of Laminin gamma 1, 2 & 3 (DLNKLNEIEGTLNKAKDEMK + MSELEERARQQRGHLHLLET + LQRKLSLLEQESQQQELQIQ) was used as the immunogen for this Laminin antibody.
Limitation:
This Laminin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human