LDHA Antibody / Lactate dehydrogenase A

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NSJ Bioreagents
Product Code: NSJ-R32543
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32543-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LDHA antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of FFPE human U-2 OS cells with LDHA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
IHC testing of FFPE human breast cancer tissue with LDHA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 6
IHC testing of FFPE mouse intestine with LDHA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 6
IHC testing of FFPE rat intestine with LDHA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Flow cytometry testing of human A549 cells with LDHA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDHA antibody.
6 / 6
Western blot testing of 1) rat spleen, 2) mouse ANA-1 and 3) human Jurkat lysate with LDHA antibody at 0.5ug/ml. Predicted/observed molecular weight ~36 kDa.
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Immunofluorescent staining of FFPE human U-2 OS cells with LDHA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human breast cancer tissue with LDHA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE mouse intestine with LDHA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat intestine with LDHA antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with LDHA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDHA antibody.
Western blot testing of 1) rat spleen, 2) mouse ANA-1 and 3) human Jurkat lysate with LDHA antibody at 0.5ug/ml. Predicted/observed molecular weight ~36 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence (FFPE): 2-4ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Lactate dehydrogenase A, also known as LDHA, is an enzyme which in humans is encoded by the LDHA gene. The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids A2-R106 from the human protein were used as the immunogen for the LDHA antibody.
Limitation:
This LDHA antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P00338