LDHB Antibody / Lactate dehydrogenase B

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NSJ Bioreagents
Product Code: NSJ-R32544
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32544-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LDHB antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of human A431 cells with LDHB antibody (red) and DAPI nuclear stain (blue).
2 / 6
IHC testing of FFPE human tonsil with LDHB antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 6
IHC testing of FFPE human brain with LDHB antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 6
IHC testing of FFPE rat spleen with LDHB antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Western blot testing of 1) human HeLa, 2) human placenta, 3) human PC-3, 4) monkey COS-7, 5) human HEK293, 6) human A549, 7) monkey kidney, 8) rat kidney, 9) rat heart, 10) mouse kidney and 11) mouse heart lysate with LDHB antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.
6 / 6
Flow cytometry testing of human A549 cells with LDHB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDHB antibody.
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Immunofluorescent staining of human A431 cells with LDHB antibody (red) and DAPI nuclear stain (blue).
IHC testing of FFPE human tonsil with LDHB antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human brain with LDHB antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat spleen with LDHB antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human placenta, 3) human PC-3, 4) monkey COS-7, 5) human HEK293, 6) human A549, 7) monkey kidney, 8) rat kidney, 9) rat heart, 10) mouse kidney and 11) mouse heart lysate with LDHB antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.
Flow cytometry testing of human A549 cells with LDHB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDHB antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
The LDHB gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids E237-L334 from the human protein were used as the immunogen for the LDHB antibody.
Limitation:
This LDHB antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
P07195