LFA-1 Antibody / CD18

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NSJ Bioreagents
Product Code: NSJ-R31562
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31562-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LFA-1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of LFA-1 antibody and Lane 1: Jurkat; 2: CEM; Predicted/Observed size: 85~95KD depending on glycosylation level
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Western blot testing of LFA-1 antibody and recombinant human protein (0.5ng)
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IHC-P: LFA-1 antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
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Immunofluorescent staining of human THP1 cells with LFA-1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Immunofluorescent staining of human tonsil tissue with LFA-1 antibody (green) at 1ug/ml and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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Flow cytometry testing of human ThP-1 cells with LFA-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LFA-1 antibody.
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Flow cytometry testing of human Raji cells with LFA-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LFA-1 antibody.
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Flow cytometry testing of human U-2 OS cells with LFA-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LFA-1 antibody.
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Western blot testing of LFA-1 antibody and Lane 1: Jurkat; 2: CEM; Predicted/Observed size: 85~95KD depending on glycosylation level
Western blot testing of LFA-1 antibody and recombinant human protein (0.5ng)
IHC-P: LFA-1 antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
Immunofluorescent staining of human THP1 cells with LFA-1 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Immunofluorescent staining of human tonsil tissue with LFA-1 antibody (green) at 1ug/ml and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of human ThP-1 cells with LFA-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LFA-1 antibody.
Flow cytometry testing of human Raji cells with LFA-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LFA-1 antibody.
Flow cytometry testing of human U-2 OS cells with LFA-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LFA-1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence/Immunocytochemistry: 1-2ug/ml, Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the LFA-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Lymphocyte function associated antigen 1, also known as CD18, is a protein that in humans is encoded by the ITGB2 gene. It is an integrin protein that belongs to the class of cell membrane glycoproteins. The beta-2 integrin chain gene is designated ITGB2 and the leukocyte antigen has been designated LFA-1/CD18. The expression of LFA-1 is increased in lymphoblastoid cells from persons with Down syndrome, consistent with the location of the gene on chromosome 21. In humans lack of LFA-1 causes Leukocyte Adhesion Deficiency, a disease defined by a lack of leukocyte extravasation from blood into tissues. Although the protein is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the 'inside-out' signaling pathways.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
3689
Immunogen:
Human partial recombinant protein (AA 404-769) was used as the immunogen for this LFA-1 antibody.
Limitation:
This LFA-1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human