LIF Antibody / Leukemia inhibitory factor / CDF

NSJ Bioreagents
Product Code: NSJ-RQ4084
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4084-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the LIF antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat brain, 2) rat heart, 3) rat liver, 4) rat kidney, 5) human HeLa, 6) human placenta, 7) human Jurkat, 8) mouse liver and 9) mouse kidney lysate with LIF antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa (unmodified), 32-62 kDa (glycosylated).

Western blot testing of 1) rat brain, 2) rat heart, 3) rat liver, 4) rat kidney, 5) human HeLa, 6) human placenta, 7) human Jurkat, 8) mouse liver and 9) mouse kidney lysate with LIF antibody at 0.5ug/ml. Predicted molecular weight ~23 kDa (unmodified), 32-62 kDa (glycosylated).

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,ELISA (Capture): 0.1-0.5ug/ml
Application Note:
Optimal dilution of the LIF antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice. This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells. LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse partial protein corresponding to amino acids S24-F203 was used as the immunogen for the LIF antibody.
Limitation:
This LIF antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P09056