Lipoamide Dehydrogenase Antibody

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NSJ Bioreagents
Product Code: NSJ-R30535
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30535-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Lipoamide Dehydrogenase antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Lipoamide Dehydrogenase antibody and Lane 1: rat liver; 2: rat brain; 3: rat ovary; 4: rat testis; 5: SMMC-7721; 6: HeLa; 7: SMMC-7721; 8: Jurkat cell lysate
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IHC-P: Lipoamide Dehydrogenase antibody testing of human breast cancer tissue
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ICC testing of MCF-7 cells
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Western blot testing of Lipoamide Dehydrogenase antibody and Lane 1: rat liver; 2: rat brain; 3: rat ovary; 4: rat testis; 5: SMMC-7721; 6: HeLa; 7: SMMC-7721; 8: Jurkat cell lysate
IHC-P: Lipoamide Dehydrogenase antibody testing of human breast cancer tissue
ICC testing of MCF-7 cells

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Lipoamide Dehydrogenase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex(BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Lipoamide Dehydrogenase (EAFREANLAASFGKSINF) was used as the immunogen for this Lipoamide Dehydrogenase antibody.
Limitation:
This Lipoamide Dehydrogenase antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P09622