Lipocalin 2 Antibody / NGAL / LCN2

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NSJ Bioreagents
Product Code: NSJ-R32053
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32053-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LCN2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) mouse lung and 2) mouse intestine lysate with LCN2 antibody. Predicted/observed molecular weight: 22-25 kDa.
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IHC testing of FFPE mouse spleen with LCN2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE rat spleen with LCN2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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IHC testing of frozen mouse spleen tissue with Lipocalin 2 antibody.
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IHC testing of frozen mouse lung tissue with Lipocalin 2 antibody.
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IHC testing of frozen rat spleen tissue with Lipocalin 2 antibody.
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IHC testing of frozen rat lung tissue with Lipocalin 2 antibody.
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Flow cytometry testing of human A431 cells with LCN2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LCN2 antibody.
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Western blot testing of 1) mouse lung and 2) mouse intestine lysate with LCN2 antibody. Predicted/observed molecular weight: 22-25 kDa.
IHC testing of FFPE mouse spleen with LCN2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat spleen with LCN2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen mouse spleen tissue with Lipocalin 2 antibody.
IHC testing of frozen mouse lung tissue with Lipocalin 2 antibody.
IHC testing of frozen rat spleen tissue with Lipocalin 2 antibody.
IHC testing of frozen rat lung tissue with Lipocalin 2 antibody.
Flow cytometry testing of human A431 cells with LCN2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LCN2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Flow cytometry : 1-3ug/million cells,ELISA : 0.1-0.5ug/ml (rat protein tested); request BSA-free format for coating
Application Note:
Optimal dilution of the LCN2 antibody should be determined by the researcher.
Description:
Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 21-198 of rat Lipocalin 2 were used as the immunogen for the LCN2 antibody.
Limitation:
This LCN2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat, Human
Uniprot #:
P30152