LYRIC Antibody (Metadherin)

Contact us
NSJ Bioreagents
Product Code: NSJ-R31751
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31751-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LYRIC antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of human 1) U-87 MG, 2) HeLa, 3) MDA-MB-453, 4) PC-3, 5) A431, 6) Caco-2 and 7) HepG2 cell lysate with LYRIC antibody. Expected molecular weight: 70-80 kDa.
2 / 6
IHC-P: LYRIC antibody testing of human breast cancer tissue
3 / 6
IHC-P: LYRIC antibody testing of rat skeletal muscle tissue
4 / 6
ICC testing of FFPE human SMMC-7721 cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 6
ICC testing of FFPE human A549 cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
6 / 6
ICC testing of FFPE human HeLa cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
❮❯

Western blot testing of human 1) U-87 MG, 2) HeLa, 3) MDA-MB-453, 4) PC-3, 5) A431, 6) Caco-2 and 7) HepG2 cell lysate with LYRIC antibody. Expected molecular weight: 70-80 kDa.
IHC-P: LYRIC antibody testing of human breast cancer tissue
IHC-P: LYRIC antibody testing of rat skeletal muscle tissue
ICC testing of FFPE human SMMC-7721 cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
ICC testing of FFPE human A549 cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
ICC testing of FFPE human HeLa cells with LYRIC antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the LYRIC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MTDH (Metadherin), also known as LYRIC or astrocyte elevated gene-1 protein (AEG-1), is a protein that in humans is encoded by the MTDH gene. It is involved in HIF-1 alpha mediated angiogenesis, interacts with SND1 and is involved in RNA-induced silencing complex (RISC), and plays a very important role in RISC and miRNA functions. LYRIC induces an oncogene called Late SV40 factor (LSF/TFCP2) which is involved in thymidylate synthase (TS) induction and DNA biosynthesis synthesis. Late SV40 factor enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9). LYRIC acts as an oncogene in melanoma, malignant glioma, breast cancer and hepatocellular carcinoma. It is highly expressed in these cancers and helps in progression and development of these cancers. It is induced by c-Myc oncogene and plays very important role in anchorage independent growth of cancer cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
92140
Immunogen:
Human partial recombinant protein (AA 101-270) was used as the immunogen for this LYRIC antibody.
Limitation:
This LYRIC antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat