M6PR Antibody / IGF2R

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4264
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4264-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the M6PR antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
IHC testing of FFPE human intestinal cancer tissue with M6PR antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 7
IHC testing of FFPE human lung cancer tissue with M6PR antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 7
IHC testing of FFPE mouse small intestine tissue with M6PR antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 7
IHC testing of FFPE rat small intestine tissue with M6PR antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 7
Western blot testing of human 1) HeLa, 2) U-87 MG, 3) HepG2, 4) 22RV1, 5) rat brain and 6) mouse brain lysate with M6PR antibody at 0.5ug/ml. Expected molecular weight: 274-300 kDa.
6 / 7
Immunofluorescent staining of FFPE human U-2 OS cells with M6PR antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 7
Flow cytometry testing of human PBM cells with M6PR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= M6PR antibody.
❮❯

IHC testing of FFPE human intestinal cancer tissue with M6PR antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human lung cancer tissue with M6PR antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse small intestine tissue with M6PR antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat small intestine tissue with M6PR antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HeLa, 2) U-87 MG, 3) HepG2, 4) 22RV1, 5) rat brain and 6) mouse brain lysate with M6PR antibody at 0.5ug/ml. Expected molecular weight: 274-300 kDa.
Immunofluorescent staining of FFPE human U-2 OS cells with M6PR antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human PBM cells with M6PR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= M6PR antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the M6PR antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Insulin-like growth factor 2 receptor, also called IGF2R, I-MPR and M6PR (mannose 6-phosphate receptor) is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids F424-R529 was used as the immunogen for the M6PR antibody.
Limitation:
This M6PR antibody is available for research use only.
Localization:
Cytoplasm (lysosome membrane)
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P11717