Mad2L1 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31695
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31695-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
After reconstitution, the Mad2L1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A431 cells with Mad2L1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human K532 cells with Mad2L1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Mad2L1 antibody.
3 / 3
Western blot testing of Mad2L1 antibody and Lane 1: 293T; 2: COLO320 lysate. Expected molecular weight: ~24 kDa.
❮❯

Immunofluorescent staining of FFPE human A431 cells with Mad2L1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human K532 cells with Mad2L1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Mad2L1 antibody.
Western blot testing of Mad2L1 antibody and Lane 1: 293T; 2: COLO320 lysate. Expected molecular weight: ~24 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Mad2L1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Mitotic spindle assembly checkpoint protein MAD2A is a protein that in humans is encoded by the MAD2L1 gene. This gene belongs to the MAD2 family. It is mapped to 4q27. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. It is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
4085
Immunogen:
Human partial recombinant protein (AA 2-205) was used as the immunogen for this Mad2L1 antibody.
Limitation:
This Mad2L1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human