MAP Kinase Antibody, Activated (Diphosphorylated ERK-1&2)

Western blot: 0.25-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: Suitable

The stated application concentrations are suggested starting amounts. Titration of the MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) may be required due to differences in protocols and secondary/substrate sensitivity.

In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein(MAP) kinase pathways. The central core of each MAP kinase(MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase(MAPKKK) phosphorylates and activates a specific MAPK kinase(MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.

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0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS

5594

A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH was used as the immunogen for this MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2).

This MAP Kinase antibody, Activated (Diphosphorylated ERK-1&2) is available for research use only.

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Human, mouse, rat, yeast