MBP Antibody

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NSJ Bioreagents
Product Code: NSJ-R30229
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30229-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MBP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of MBP antibody and mouse brain tissue lysate. Multiple isoforms may be visualized from 20~37 kDa.
2 / 15
IHC-P: MBP antibody testing of rat brain tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
3 / 15
Immunofluorescent staining of FFPE mouse brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 15
Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 15
Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
6 / 15
Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 15
Western blot testing of MBP antibody with 1) mouse brain and 2) rat brain lysate. Multiple isoforms may be visualized from 20~37 kDa.
8 / 15
Immunofluorescent staining of FFPE rat brain with MBP antibody (red), anti-GFAP mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
9 / 15
IHC-P: MBP antibody testing of mouse hippocampus tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
10 / 15
IHC-P: MBP antibody testing of mouse cerebral cortex tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
11 / 15
IHC-P: MBP antibody testing of mouse brain tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
12 / 15
IHC-P: MBP antibody testing of mouse cerebellum tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
13 / 15
IHC-P: MBP antibody testing of rat hippocampus tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
14 / 15
IHC-P: MBP antibody testing of rat cerebral cortex tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
15 / 15
IHC-P: MBP antibody testing of rat cerebellum tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
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Western blot testing of MBP antibody and mouse brain tissue lysate. Multiple isoforms may be visualized from 20~37 kDa.
IHC-P: MBP antibody testing of rat brain tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE mouse brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat brain with MBP antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of MBP antibody with 1) mouse brain and 2) rat brain lysate. Multiple isoforms may be visualized from 20~37 kDa.
Immunofluorescent staining of FFPE rat brain with MBP antibody (red), anti-GFAP mAb (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: MBP antibody testing of mouse hippocampus tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of mouse cerebral cortex tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of mouse brain tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of mouse cerebellum tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of rat hippocampus tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of rat cerebral cortex tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.
IHC-P: MBP antibody testing of rat cerebellum tissue. HIER: boil tissue sections in pH8 EDTA buffer for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Myelin Basic Protein (KLGGRDSRSGSPMARR) was used as the immunogen for this MBP antibody (100% homologous in human, mouse and rat).
Limitation:
This MBP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P02686