MCAK Antibody

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NSJ Bioreagents
Product Code: NSJ-R31643
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31643-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MCAK antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with MCAK antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
Flow cytometry testing of human K562 cells with MCAK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCAK antibody.
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IHC-P: MCAK antibody testing of mouse testis tissue
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IHC-P: MCAK antibody testing of rat testis tissue
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IHC-P: MCAK antibody testing of rat thymus tissue
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Western blot testing of MCAK antibody and Lane 1: mouse testis; 2: human HeLa; 3: (h) MM231; 4: (h) MM453; 5: (h) SKOV lysate. Predicted molecular weight ~81 kDa.
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Western blot testing of MCAK antibody and recombinant human protein (0.5ng)
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Immunofluorescent staining of FFPE human U-2 OS cells with MCAK antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human K562 cells with MCAK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCAK antibody.
IHC-P: MCAK antibody testing of mouse testis tissue
IHC-P: MCAK antibody testing of rat testis tissue
IHC-P: MCAK antibody testing of rat thymus tissue
Western blot testing of MCAK antibody and Lane 1: mouse testis; 2: human HeLa; 3: (h) MM231; 4: (h) MM453; 5: (h) SKOV lysate. Predicted molecular weight ~81 kDa.
Western blot testing of MCAK antibody and recombinant human protein (0.5ng)

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MCAK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Kinesin-like protein, also called Mitotic centromere associated kinesin (MCAK), is a protein that in humans is encoded by the KIF2C gene. It is mapped to 1p34.1. The protein encoded by this gene is a member of kinesin-like protein family. Most proteins of this family are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein acts to regulate microtubule dynamics in cells and is important for anaphase chromosome segregation and may be required to coordinate the onset of sister centromere separation. MCAK uses microtubule depolymerizing activity to correct improper microtubule attachments at kinetochores.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
11004
Immunogen:
Human partial recombinant protein (AA 531-725) was used as the immunogen for this MCAK antibody.
Limitation:
This MCAK antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat