MCM2 Antibody

Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml

The stated application concentrations are suggested starting amounts. Titration of the MCM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Minichromosome maintenance, S. cerevisiae, homolog of, 2, also known as MITOTIN, CDCL1 or BM28, is a human nuclear protein that plays an important role in two crucial steps of the cell cycle, namely, onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. The MCM2 gene is mapped to 3q21.3. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex(pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. In the G0 stage, the MCM2 and MCM5 proteins were much less abundant than the MCM7 and MCM3 proteins, which suggests that the MCM proteins are not present in stoichiometric amounts and that only a proportion of these molecules actively participate in cell cycle regulation as part of MCM complexes.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

An amino acid sequence from the C-terminus of human MCM2 (NKFSHDLKRKMILQQF) was used as the immunogen for this MCM2 antibody.

This MCM2 antibody is available for research use only.

Antigen affinity

Human, Mouse, Rat

P49736