MCM3 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R30735
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30735-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MCM3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 9
Immunofluorescent staining of FFPE human U-2 OS cells with MCM3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 9
IHC-P: MCM3 antibody testing of human lung cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 9
IHC-P: MCM3 antibody testing of rat intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 9
ICC testing of HeLa cells
5 / 9
IHC-P: MCM3 antibody testing of mouse intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.
6 / 9
IHC-P: MCM3 antibody testing of rat intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.
7 / 9
Western blot testing of human 1) HeLa and 2) Jurkat cell lysate. Expected molecular weight: 90~115 kDa.
8 / 9
Western blot testing of mouse 1) NIH-3T3 and 2) HEPA1-6 cell lysate. Expected molecular weight: 90~115 kDa.
9 / 9
Flow cytometry testing of human SiHa cells with MCM3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM3 antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with MCM3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: MCM3 antibody testing of human lung cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: MCM3 antibody testing of rat intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.
ICC testing of HeLa cells
IHC-P: MCM3 antibody testing of mouse intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: MCM3 antibody testing of rat intestine tissue. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa and 2) Jurkat cell lysate. Expected molecular weight: 90~115 kDa.
Western blot testing of mouse 1) NIH-3T3 and 2) HEPA1-6 cell lysate. Expected molecular weight: 90~115 kDa.
Flow cytometry testing of human SiHa cells with MCM3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM3 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml (1),Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MCM3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Minichromosome maintenance, S. cerevisiae, homolog of, 3, also called RLFB or P1 Protein, is a protein that in humans is encoded by the MCM3 gene. MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human MCM3 (REAQRDYLDFLDDEE) was used as the immunogen for this MCM3 antibody (100% homologous in human, mouse and rat).
Limitation:
This MCM3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P25205