MCM6 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30862
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30862-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MCM6 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of MCM6 antibody and Lane 1: U87; 2: COLO320; 3: HeLa; 4: MCF-7; 5: Jurkat cell lysate
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IHC-P: MCM6 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
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IHC-P: MCM6 antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
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ICC staining of human HeLa cells with MCM6 antibody.
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IHC staining of frozen human placenta tissue with MCM6 antibody.
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IHC staining of FFPE rat intestine with MCM6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human A431 cells with MCM6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of mouse 1) small intestine and 2) spleen lysate with MCM6 antibody. Predicted molecular weight: ~93 kDa.
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Flow cytometry testing of human SiHa cells with MCM6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM6 antibody.
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Western blot testing of MCM6 antibody and Lane 1: U87; 2: COLO320; 3: HeLa; 4: MCF-7; 5: Jurkat cell lysate
IHC-P: MCM6 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: MCM6 antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
ICC staining of human HeLa cells with MCM6 antibody.
IHC staining of frozen human placenta tissue with MCM6 antibody.
IHC staining of FFPE rat intestine with MCM6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with MCM6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of mouse 1) small intestine and 2) spleen lysate with MCM6 antibody. Predicted molecular weight: ~93 kDa.
Flow cytometry testing of human SiHa cells with MCM6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MCM6 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MCM6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Minichromosome maintenance, s. pombe, homolog of, 6 is a protein that in humans is encoded by the MCM6 gene. It is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM genes were originally identified in yeast defective in minichromosome maintenance and have since been shown to play roles in the progression of the cell cycle; many are cell division control genes. MCM6 has recently been shown to interact strongly Cdt1 at defined residues by mutating these target residues. Wei et al. observed lack of Cdt1 recruitment of MCM2-7 to the pre-RC. An approximately 200-kb region surrounding the C/T(-13910) polymorphism in MCM6 intron 13 functioned as an enhancer of the lactase gene promoter in intestinal cell culture.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human MCM6 (ESEDFIVEQYKHLRQRD) was used as the immunogen for this MCM6 antibody.
Limitation:
This MCM6 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q14566