MED15 Antibody / PCQAP

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NSJ Bioreagents
Product Code: NSJ-R32948
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32948-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MED15 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) U-87MG, 2) U-2 OS, 3) HeLa, 4) 22RV1, 5) K562, 6) rat liver and 7) mouse thymus lysate with MED15 antibody at 0.5ug/ml. Predicted molecular weight ~87 kDa.
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IHC testing of FFPE human colon cancer tissue with MED15 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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IHC testing of FFPE human lung cancer tissue with MED15 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 9
IHC testing of FFPE human placental tissue with MED15 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 9
IHC testing of FFPE mouse brain tissue with MED15 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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IHC testing of FFPE rat brain tissue with MED15 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
7 / 9
Immunofluorescent staining of human U-2 OS cells with MED15 antibody (red) and DAPI nuclear stain (blue).
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Flow cytometry testing of human U-2 OS cells with MED15 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MED15 antibody.
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Western blot testing of human 1) 293T, 2) K562 and 3) HeLa cell lysate with MED15 antibody at 0.5ug/ml. Predicted molecular weight ~87 kDa.
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Western blot testing of human 1) U-87MG, 2) U-2 OS, 3) HeLa, 4) 22RV1, 5) K562, 6) rat liver and 7) mouse thymus lysate with MED15 antibody at 0.5ug/ml. Predicted molecular weight ~87 kDa.
IHC testing of FFPE human colon cancer tissue with MED15 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human lung cancer tissue with MED15 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human placental tissue with MED15 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse brain tissue with MED15 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat brain tissue with MED15 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Immunofluorescent staining of human U-2 OS cells with MED15 antibody (red) and DAPI nuclear stain (blue).
Flow cytometry testing of human U-2 OS cells with MED15 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MED15 antibody.
Western blot testing of human 1) 293T, 2) K562 and 3) HeLa cell lysate with MED15 antibody at 0.5ug/ml. Predicted molecular weight ~87 kDa.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA (recombinant human protein): 0.1-0.5ug/ml (BSA-free format available),Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the MED15 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Mediator of RNA polymerase II transcription subunit 15, also known as Gal11, Spt13 in yeast and PCQAP, ARC105, or TIG-1 in humans is a protein encoded by the MED15 gene. The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids M1-A285 was used as the immunogen for the MED15 antibody.
Limitation:
This MED15 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q96RN5