MED18 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32989
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32989-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MED18 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) A431, 2) K562, 3) U-2 OS, 4) HL60, 5) rat liver and 6) mouse kidney lysate with MED18 antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
2 / 10
IHC testing of FFPE human lung cancer tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 10
IHC testing of FFPE human intestinal cancer tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 10
IHC testing of FFPE human breast cancer tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 10
IHC testing of FFPE mouse brain tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
6 / 10
IHC testing of FFPE mouse small intestine tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
7 / 10
IHC testing of FFPE rat brain tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
8 / 10
IHC testing of FFPE rat small intestine tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
9 / 10
Flow cytometry testing of human A431 cells with MED18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MED18 antibody.
10 / 10
Immunofluorescent staining of FFPE human A431 cells with MED18 antibody (red) and DAPI (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
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Western blot testing of human 1) A431, 2) K562, 3) U-2 OS, 4) HL60, 5) rat liver and 6) mouse kidney lysate with MED18 antibody at 0.5ug/ml. Predicted molecular weight ~24 kDa.
IHC testing of FFPE human lung cancer tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human intestinal cancer tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human breast cancer tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse brain tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse small intestine tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat brain tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat small intestine tissue with MED18 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Flow cytometry testing of human A431 cells with MED18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MED18 antibody.
Immunofluorescent staining of FFPE human A431 cells with MED18 antibody (red) and DAPI (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml,Direct ELISA (recombinant human protein): 0.1-0.5ug/ml (BSA-free format available)
Application Note:
Optimal dilution of the MED18 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Using in vitro-translated epitope-tagged proteins for protein-binding assays, it is found that MED18 directly interacted with the Mediator subunit TRFP (MED20). The MED18-TRFP heterodimer could also be coimmunoprecipitated from cotransfected insect cells. The MED18 gene was mapped to chromosome 1p35.3 based on an alignment of the MED18 sequence with the genomic sequence.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids M1-M208 was used as the immunogen for the MED18 antibody.
Limitation:
This MED18 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9BUE0