MEK1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30445
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30445-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MEK1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of MEK1 antibody and Lane 1: rat skeletal muscle; 2: rat kidney; 3: (h) CEM; 4: (h) COLO20 cell lysate
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IHC-P: MEK1 antibody testing of human lung cancer tissue
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IHC-P: MEK1 antibody testing of human breast cancer tissue
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ICC testing of MCF-7 cells
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Western blot testing of MEK1 antibody and Lane 1: rat skeletal muscle; 2: rat kidney; 3: (h) CEM; 4: (h) COLO20 cell lysate
IHC-P: MEK1 antibody testing of human lung cancer tissue
IHC-P: MEK1 antibody testing of human breast cancer tissue
ICC testing of MCF-7 cells

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MEK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Dual specificity mitogen-activated protein kinase kinase 1 is an enzyme that in humans is encoded by the MAP2K1 gene. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein(MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act as an integration point for multiple biochemical signals. MEK1 lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon activation by a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Rampoldi et al.(1997) localized the MAP2K1 gene to 15q22.1-q22.33.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human MEK1 (KQLMVHAFIKRSDAE) was used as the immunogen for this MEK1 antibody (100% homologous in human, mouse and rat).
Limitation:
This MEK1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q02750