Mesothelin Antibody

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NSJ Bioreagents
Product Code: NSJ-R31700
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31700-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Mesothelin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Mesothelin antibody and recombinant human protein (0.5ng)
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IHC-P: Mesothelin antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
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Flow cytometry testing of human SiHa cells with Mesothelin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Mesothelin antibody.
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Western blot testing of Mesothelin antibody and recombinant human protein (0.5ng)
IHC-P: Mesothelin antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
Flow cytometry testing of human SiHa cells with Mesothelin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Mesothelin antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,ELISA: 0.1-0.5ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Mesothelin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Mesothelin, also known as MSLN or MPF, is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, Megakaryocyte potentiating factor and Mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
10232
Immunogen:
Human partial recombinant protein (AA 306-576) was used as the immunogen for this Mesothelin antibody.
Limitation:
This Mesothelin antibody is available for research use only.
Localization:
Cell membrane & secreted
Purity:
Antigen affinity
Species Reactivity :
Human