MFGE8 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31046
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31046-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MFGE8 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of MEGF8 antibody and recombinant protein; Lane 1: human protein 10ng; 2: 5ng; 3: 2.5ng; 4: 1.25ng~
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IHC-P: MFGE8 antibody testing of human breast cancer tissue
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Western blot testing of MEGF8 antibody and recombinant protein; Lane 1: human protein 10ng; 2: 5ng; 3: 2.5ng; 4: 1.25ng~
IHC-P: MFGE8 antibody testing of human breast cancer tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MFGE8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Milk Fat Globule-Egf Factor 8, also called Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. It is a secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al.(1997) mapped the gene to chromosome 15q25. Hanayama et al.(2002) found that it is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. When engaged by phospholipids, it bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al.(2007) showed that MFGE8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that the protein promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human MFGE8 (NGHEFDFIHDVNKKHK) was used as the immunogen for this MFGE8 antibody.
Limitation:
This MFGE8 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q08431