MGMT Antibody

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NSJ Bioreagents
Product Code: NSJ-R31679
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31679-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MGMT antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human MCF7 cells with MGMT antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
ICC staining of FFPE human MCF7 cells with MGMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC-P: MGMT antibody testing of human placenta tissue. HIER: steam section in pH6 citrate buffer for 20 min.
4 / 6
Western blot testing of MGMT antibody and human samples 1: HeLa; 2: placenta; 3: Jurkat. Predicted molecular weight: ~23 kDa.
5 / 6
Western blot testing of MGMT antibody and recombinant human protein (0.5ng).
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Flow cytometry testing of human ThP-1 cells with MGMT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MGMT antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with MGMT antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
ICC staining of FFPE human MCF7 cells with MGMT antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC-P: MGMT antibody testing of human placenta tissue. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of MGMT antibody and human samples 1: HeLa; 2: placenta; 3: Jurkat. Predicted molecular weight: ~23 kDa.
Western blot testing of MGMT antibody and recombinant human protein (0.5ng).
Flow cytometry testing of human ThP-1 cells with MGMT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MGMT antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
O6-alkylguanine DNA alkyltransferase (also known as AGT, MGMT or AGAT) is a protein that in humans is encoded by the MGMT gene. This gene is crucial for genome stability. The protein repairs the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine and prevents mismatch and errors during DNA replication and transcription. It has been found that methylation of the MGMT promoter in the tumor was associated with longer survival.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
4255
Immunogen:
Human partial recombinant protein (AA 2-207) was used as the immunogen for this MGMT antibody.
Limitation:
This MGMT antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human