MIF Antibody

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NSJ Bioreagents
Product Code: NSJ-R30231
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30231-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MIF antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human breast cancer with c-Kit antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE rat liver tissue with c-Kit antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
3 / 3
Western blot testing of MIF antibody and Lane 1: human HeLa; 2: (h) COLO320; 3: (h) MDA-MB-231 cell lysate. Predicted molecular weight ~13 kDa.
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IHC staining of FFPE human breast cancer with c-Kit antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with c-Kit antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Western blot testing of MIF antibody and Lane 1: human HeLa; 2: (h) COLO320; 3: (h) MDA-MB-231 cell lysate. Predicted molecular weight ~13 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Macrophage migration inhibitory factor, MIF, is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. Localization of the human gene for macrophage migration inhibitory factor(MIF) to chromosome 22q11.2 MIF plays a critical role in inflammatory diseases and atherogenesis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human MIF (NYYDMNAANVGWNNSTFA) was used as the immunogen for this MIF antibody.
Limitation:
This MIF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P14174