MIF Antibody

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NSJ Bioreagents
Product Code: NSJ-R31687
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31687-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
The lyophilized MIF antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freeze/thaws.

Images

1 / 5
Immunofluorescent staining of FFPE human HeLa cells with MIF antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE human liver cancer tissue with MIF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human lung cancer tissue with MIF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) Jurkat, 2) K562, 3) HeLa and 4) HepG2 cell lysat with MIF antibody. Predicted molecular weight ~13 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human Jurkat cells with MIF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MIF antibody.
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Immunofluorescent staining of FFPE human HeLa cells with MIF antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human liver cancer tissue with MIF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with MIF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Jurkat, 2) K562, 3) HeLa and 4) HepG2 cell lysat with MIF antibody. Predicted molecular weight ~13 kDa.
Flow cytometry testing of fixed and permeabilized human Jurkat cells with MIF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MIF antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
Variations in secondary/substrate sensitivities and test protocols may require the MIF antibody to be titrated for optimal performance.
Description:
Macrophage migration inhibitory factor (MMIF), also known as GIF, is a protein that in humans is encoded by the MIF gene. It is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. The gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. MIF plays a critical role in inflammatory diseases and atherogenesis. It is also involved in cell-mediated immunity and immunoregulation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
4282
Immunogen:
Human partial recombinant protein (AA 2-115) was used as the immunogen for this MIF antibody.
Limitation:
This MIF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human