MMP16 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30291
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30291-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MMP16 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of frozen human placental tissue with MMP16 antibody.
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IHC staining of frozen rat intestine tissue with MMP16 antibody.
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IHC staining of FFPE human rectal cancer tissue with MMP16 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
4 / 4
Western blot testing of MMP16 antibody and human heart tissue lysate. Predicted molecular weight: ~70 kDa (long form), ~53 kDa (short form).
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IHC staining of frozen human placental tissue with MMP16 antibody.
IHC staining of frozen rat intestine tissue with MMP16 antibody.
IHC staining of FFPE human rectal cancer tissue with MMP16 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Western blot testing of MMP16 antibody and human heart tissue lysate. Predicted molecular weight: ~70 kDa (long form), ~53 kDa (short form).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MMP16 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The matrix metalloproteinase 16(MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human MMP16 (YTVFQFKRKGTPRHILY) was used as the immunogen for this MMP16 antibody (100% homologous in human, mouse and rat).
Limitation:
This MMP16 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P51512